YES!! This is one of our favorite applications. In the wintertime, those of us with raised houses suffer from cold, moist wood floors & in the summertime, we lose much of our conditioned air through cracks in our floors. Applying our product under the wood floors or under subflooring not only creates an air seal & insulates the floor, but it also seals all the cracks, crevasses, & holes where rodents, roaches, & spiders enter our homes. The foam will also protect your wood floors from soil moisture rising from the ground, which can slowly rot your wood. Installing fiberglass batts under your floors can be a huge mistake because they tend to drop a few inches, creating an air gap where moisture can penetrate & wreak havoc on your wood, not to mention creating a more pleasant environment for termites & rodents. Although there are differing opinions on closed-cell vs. open-cell foam for a subfloor application, our experience indicates that closed-cell foam is a preferable (but more expensive) product for a subfloor in our climate. Most building scientists & manufacturers agree with this opinion. Although we have never encountered any issues with open-cell foam underneath a raised house (& we think it is much better than fiberglass), closed-cell foam is more preferable in our opinion. I (Kirk, the owner) have closed cell foam under my own house, but have open cell foam under a few of my rental properties.